Oral Presentation 7th Modern Solid Phase Peptide Synthesis & Its Applications Symposium 2019

Peptide synthesis workflow: identify and overcome its bottlenecks (#5)

Cedric Rentier 1
  1. Biotage Japan Ltd., Koto-ku, Tokyo, Japan

Peptide synthesis workflow is a multi-step process which involves synthesis, purification and evaporation to obtain the desired peptide product. This demands that the steps from amino acid building blocks to final purified peptide product proceed as efficiently and smoothly as possible. In the last couple of years automated SPPS was optimized in various ways, such as: reduce its time as much as possible, increase the scale of a single batch, obtain crude purities as high as possible, or perform multiple parallel syntheses.

However, purification and evaporation issues can dramatically impact the efficiency of the workflow and are the cause of many bottlenecks often disregarded by scientists.

Preparative reversed-phase HPLC has normally been the method of choice for purification of synthetic peptides, but is limited by small loading amounts, long separation times, poor recoveries and high costs. Is there an alternative?

Similarly, freeze drying of peptides, despite being an absolutely crucial step for the whole process, is generally accepted as time-consuming and requires special equipment as well as a reliable access to liquid nitrogen supply. Are there any other ways?

Scientists want to get their peptides always faster, how can you put together the different steps of the process in the most time-efficient possible way?

This presentation will feature strategies to improve the peptide synthesis workflow, which enable the synthesis of highly pure crude peptides, demonstrate alternative techniques to rapidly purify crude peptide mixtures and highlight methods to rapidly concentrate solutions along the entire peptide synthesis workflow.